要点:CITE-seq 的膜蛋白marker的意义;批量绘制 FeaturePlot。
- T: CD5, CD7, TNFRSF8(CD30)?, NCAM1(CD56)?
- mono: CD33, ANPEP(CD13), FCGR1A(CD64), FUT4(CD15)?, IL3RA(CD123)
- B: MME(CD10), CD38
- ery: TFRC(CD71),
- HSC: CD34, KIT(CD117),
1,feature plot with no axis
2. Code
FeaturePlot(scObj, features =c("CD3D", "CD8A", "GZMB", "CCR7", "CCL5", "CD5","CD7", "HLA-DRA","LYZ", "CD33", "ANPEP", "FCGR1A", "IL3RA","CD79A","CD79B","VPREB1", "IGJ","MME","CD38","HBA1", "GATA1", "TFRC",#CD71"CD34", "SOCS2", "GAPDH"), cols = c("lightgrey", 'red'),ncol = 5 ) & NoLegend() & NoAxes() & theme(panel.border = element_rect(color = "black", size = 1))